| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1466-2 | ||||
Resumo:The fungi that cause white rot are known for their ability to produce metabolites of industrial interest, mainly ligninolytic enzymes. However, the production of biosurfactants by these organisms is poorly referenced in the literature and has agricultural, environmental, and industrial cleaning applications. Thus, this study aimed to evaluate the production of biosurfactants by the fungus Lentinus sp. CCIBT 2611 in semisolid fermentation (FSS) consisting of green coconut fiber supplemented with soybean meal. The FSS was conducted in glass bottles with a capacity of 500 mL and consisted of 30 g of crushed green coconut fiber supplemented with 0.33 g of soybean flour with C/N of 90, and humidity of 70% (Bu). Ten disks (0.5 cm diameter) of mycelial growth of Lentinus sp. in BDA medium were used as inoculum. Biosurfactant production was monitored for 21 days. The crude extract was obtained by adding 50 mL of 50 mM sodium acetate buffer, pH 5 to FSS. The contents of the flasks were homogenized manually for 5 min followed by stirring in a shaker for 1 h (120 rpm, 25 ºC) and vacuum filtration using filter paper. Biosurfactant production was evaluated every 3 days by the emulsion index (IE) calculation. For this purpose, 2 mL of crude extract and 1 mL of kerosene were added to test tubes and stirred in a vortex for 2 min. IE was calculated by dividing the emulsion height formed by the total liquid height. The emulsion stability was evaluated at intervals of 24h after the beginning of the test, comparing the ies. Biosurfactant production was identified throughout the period evaluated with IE values ranging between 35% and 42.2%. The extracts obtained with 15, 18, and 21 days of FSS were not stable after 24 h. The less stable emulsion was obtained with 3 days of FSS, while the maximum stability (95%) was verified in the extract obtained at 9 days of FSS. After 24 days the emulsion of the extract obtained on the ninth day of FSS maintained about 50% of the height measured at the beginning of the trial. The results indicate that green coconut fiber is a promising substrate for producing stable biosurfactants by Lentinus sp. showing the biotechnological potential of that industrial metabolite. Palavras-chave: basiodiomycete, green coconut fiber, metabolite, semi-solid fermentation Agência de fomento:Federal University of Campina Grande | |||||